Evaluation of the Genetic Structure of Some Accessions Belonging to Onobrychis spp. Using Microsatellite DNA Markers

Turkey is in a very convenient position for animal husbandry terms of both natural resources and ecological conditions. Forage crops, which has important place agricultural activities, the insurance plant production. Sainfoin perennial forage legume species that grown northern temperate regions world from Mediterranean region Caucasus, to Central Asia. In this study genetic diversity 100 genotypes representing 44 accessions 18 different Onobrychis (O. arenaria subsp. arenaria, O. inermis, petraea, cyri, iberica, altissima, vassilczenkoi, conferta argentea, alba laconica, biebersteinii, grandis, kachetica, kemulariae, oxyodonta, megataphros, pallasii, spp., viciifolia) were evaluated using 8 simple sequence repeat (microsatellite) markers. Based on results, OVK036, OVK094, OVK125, OVM033, OVK161, OVK046, OVM061, OVK174 loci polymorphic. The observed number alleles per SSR locus ranged 6 21 (mean 11.625). Maximum allele frequency 0.51 0.93 with mean value 0.73. PIC 0.124 0.244. polymorphism information content was 0.188. Genetic coefficients according UPGMA 0.000 0.9375. Cluster analysis divided sainfoin into two main groups (Cluster-I Cluster-II). All diploid (except 1 genotype) used formed separate group within Cluster-I. results revealed markers are useful molecular characterization assessing accessions. obtained variability studied certain provided significant about structure could be as parental lines breeding programs.